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Preparation of the Rat Vocal Fold for Neuromuscular Analyses

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparation of the Rat Vocal Fold for Neuromuscular Analyses

Preparation of the Rat Vocal Fold for Neuromuscular Analyses

DOI:

10.3791/61327-v

•

07:17 min

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May 15, 2020

•

Charles Lenell², Adrianna C. Shembel, Aaron M. Johnson

¹NYU Voice Center, Department of Otolaryngology-Head & Neck Surgery,New York University School of Medicine, ²Communicative Sciences and Disorders,New York University

Chapters

00:05Introduction
00:54Rat Larynx Dissection
02:25Laryngeal Tissue Fixation and Flash-Freezing
03:33Hemilarynx Cross-Sectional Plane Cryosectioning
04:50Hemilarynx Longitudinal Plane Cryosectioning
05:40Results: Representative Rat Vocal Fold Neuromuscular Analyses
06:43Conclusion

Summary

Automatic Translation

This protocol describes methods used to prepare rat vocal folds for histochemical neuromuscular study.

Tags

Keywords: Rat Vocal Fold Laryngeal Analysis Neuromuscular Analysis Cryosectioning Intrinsic Laryngeal Muscles Thyroarytenoid Muscle Larynx Dissection Formaldehyde Fixation Sucrose Cryoprotection

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