Journal
/
Neuroscience
/
Collection of Frozen Rodent Brain Regions for Downstream Analyses
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Collection of Frozen Rodent Brain Regions for Downstream Analyses

Collection of Frozen Rodent Brain Regions for Downstream Analyses

DOI:
10.3791/60474-v

•

07:06 min

•

April 23, 2020

•

, , ,
1Department of Psychological and Brain Sciences, Gill Center for Biomolecular Research,Indiana University

Chapters

  • 00:04Introduction
  • 00:46Setup and Preparation
  • 03:39Dissection
  • 04:36Results: Analysis of RNA and Protein with the Frozen Brain Dissection Method
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure describes the collection of discrete frozen brain regions to obtain high-quality protein and RNA using inexpensive and commonly available tools.

Tags

Frozen Brain RegionsProtein And RNA AnalysisBrain DissectionBrain MatrixLiquid NitrogenIsopentaneDry IceGlass PlateForcepsRazor BladesBrain LandmarksCD-1 Wild Type Mice

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Simultaneous fMRI and Electrophysiology in the Rodent Brain

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Morphometric Analyses of Retinal Sections

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Personalized Needles for Microinjections in the Rodent Brain

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Read Article