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Preparation of the Rat Vocal Fold for Neuromuscular Analyses
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JoVE Journal Neuroscienze
Preparation of the Rat Vocal Fold for Neuromuscular Analyses

Preparation of the Rat Vocal Fold for Neuromuscular Analyses

DOI:
10.3791/61327-v

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07:17 min

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May 15, 2020

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, ,
1NYU Voice Center, Department of Otolaryngology-Head & Neck Surgery,New York University School of Medicine, 2Communicative Sciences and Disorders,New York University

Capitoli

  • 00:05Introduction
  • 00:54Rat Larynx Dissection
  • 02:25Laryngeal Tissue Fixation and Flash-Freezing
  • 03:33Hemilarynx Cross-Sectional Plane Cryosectioning
  • 04:50Hemilarynx Longitudinal Plane Cryosectioning
  • 05:40Results: Representative Rat Vocal Fold Neuromuscular Analyses
  • 06:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes methods used to prepare rat vocal folds for histochemical neuromuscular study.

Tags

Keywords: Rat Vocal FoldLaryngeal AnalysisNeuromuscular AnalysisCryosectioningIntrinsic Laryngeal MusclesThyroarytenoid MuscleLarynx DissectionFormaldehyde FixationSucrose Cryoprotection

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