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Canlı Isı Stresli Drosophila Embriyolarında İntranükleer Akin Çubuklarının Görüntülenmesi
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May 15, 2020
DOI:
1Integrative Molecular and Biomedical Sciences,Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Summary
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Bu protokolün amacı, Rhodamine konjuge globüler aksini Drosophila embriyolarına enjekte etmek ve ısı stresini takiben intranükleer aksin çubuk tertibatını görmektir.
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Biel, N., Figard, L., Sokac, A. M. Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos. J. Vis. Exp. (159), e61297, doi:10.3791/61297 (2020).
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