Dergi
/
Gelişim Biyolojisi
/
Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

3,302 Views

•

07:57 min

•

May 15, 2020

DOI:

10.3791/61297-v

DOI:
10.3791/61297-v

•

07:57 min
May 15, 2020

•

3 Views
, ,
1Integrative Molecular and Biomedical Sciences,Baylor Tıp Fakültesi, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor Tıp Fakültesi

Özet

Automatically generated

The goal of this protocol is to inject Rhodamine-conjugated globular actin into Drosophila embryos and image intranuclear actin rod assembly following heat stress.

Explore More Videos

Intranuclear Actin RodsLive Heat Stressed Drosophila EmbryosMicroinjectionActin Stress ResponseActin Rod AssemblyMutant ScreenStress ConditionsEmbryo HandlingEmbryo PositioningEmbryo MountingG-actin RedApple Juice AgarDechorionationEmbryo TransferEmbryo AlignmentEmbryo GlueCoverslip Mounting

Read Article

Cite this Article

Biel, N., Figard, L., Sokac, A. M. Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos. J. Vis. Exp. (159), e61297, doi:10.3791/61297 (2020).

Download .ris file

Copy

Share Video

.

Copy