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Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
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May 15, 2020
DOI:
1Integrative Molecular and Biomedical Sciences,Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Summary
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L'obiettivo di questo protocollo è quello di iniettare l'actina globulare coniugata con rhodamina negli embrioni di Drosophila e nell'assemblaggio dell'asta di actina intranucleare dell'immagine a seguito dello stress da calore.
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Biel, N., Figard, L., Sokac, A. M. Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos. J. Vis. Exp. (159), e61297, doi:10.3791/61297 (2020).
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