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Nichtradioaktiver Test zur Messung der Polynukleotid-Phosphorylierung kleiner Nukleotidsubstrate
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Biochemistry
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JoVE Journal Biochemistry
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Please note that all translations are automatically generated. Click here for the English version.

Nichtradioaktiver Test zur Messung der Polynukleotid-Phosphorylierung kleiner Nukleotidsubstrate

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4,502 Views

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06:49 min

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May 08, 2020

DOI:

10.3791/61258-v

DOI:
10.3791/61258-v

•

06:49 min
May 08, 2020

•

2 Views
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1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Summary

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Dieses Protokoll beschreibt einen nicht radioaktiven Test zur Messung der Kinaseaktivität von Polynukleotid-Kinasen (PNKs) auf kleinen DNA- und RNA-Substraten.

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Cite this Article

Pillon, M. C., Stanley, R. E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (159), e61258, doi:10.3791/61258 (2020).

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