ジャーナル
/
生化学
/
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

DOI:
10.3791/61258-v

•

06:49 min

•

May 08, 2020

•

,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health (NIH)

章

  • 00:05Introduction
  • 00:29In Vitro RNA Kinase Reaction
  • 01:27Gel Electrophoresis
  • 04:53Results: Quantification of RNA Phosphorylation
  • 06:06Conclusion

概要

自動翻訳

自動翻訳

This protocol describes a nonradioactive assay to measure kinase activity of polynucleotide kinases (PNKs) on small DNA and RNA substrates.

タグ

Polynucleotide KinasePhosphorylationDNARNASubstrateNonradioactive AssayAcrylamide GelDenaturing GelUreaTBE BufferRNA Enzyme Kinase ReactionsATP SubstrateQuenchingDownstream Analysis

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Oligopeptide Competition Assay for Phosphorylation Site Determination

Quantitative Immunofluorescence to Measure Global Localized Translation

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Read Article