Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
4,502 Views
•
06:49 min
•
May 08, 2020
DOI:
Summary
Automatically generated
This protocol describes a nonradioactive assay to measure kinase activity of polynucleotide kinases (PNKs) on small DNA and RNA substrates.
Read Article
Cite this Article
Pillon, M. C., Stanley, R. E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (159), e61258, doi:10.3791/61258 (2020).
Copy