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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

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Biochimie

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Journal JoVE Biochimie

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

4,486 Views

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06:49 min

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May 08, 2020

DOI:

10.3791/61258-v

DOI:

10.3791/61258-v

•

06:49 min

May 08, 2020

•

1 Views

Monica C. Pillon, Robin E. Stanley

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Tags

Polynucleotide Kinase Phosphorylation DNA RNA Substrate Nonradioactive Assay Acrylamide Gel Denaturing Gel Urea TBE Buffer RNA Enzyme Kinase Reactions ATP Substrate Quenching Downstream Analysis

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