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小ヌクレオチド基質のポリヌクレオチドリン酸化を測定する非放射性アッセイ
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
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小ヌクレオチド基質のポリヌクレオチドリン酸化を測定する非放射性アッセイ

4,486 Views

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06:49 min

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May 08, 2020

DOI:

10.3791/61258-v

DOI:
10.3791/61258-v

•

06:49 min
May 08, 2020

•

1 Views
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1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Tags

Polynucleotide KinasePhosphorylationDNARNASubstrateNonradioactive AssayAcrylamide GelDenaturing GelUreaTBE BufferRNA Enzyme Kinase ReactionsATP SubstrateQuenchingDownstream Analysis

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Cite this Article

Pillon, M. C., Stanley, R. E. Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates. J. Vis. Exp. (159), e61258, doi:10.3791/61258 (2020).

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