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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

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06:49 min

May 08, 2020

DOI:

06:49 min
May 08, 2020

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Summary

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This protocol describes a nonradioactive assay to measure kinase activity of polynucleotide kinases (PNKs) on small DNA and RNA substrates.

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