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用于微CT成像的成人和早期产后小鼠肺的血管铸造
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging
DOI:

09:00 min

June 20, 2020

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Chapters

  • 00:04Introduction
  • 00:52Pulmonary Artery (PA) Catheterization and Blood Perfusion
  • 03:18Tracheostomy and Lung Inflation
  • 04:59Casting the Vasculature
  • 06:44Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
  • 08:24Conclusion

Summary

Automatic Translation

该技术的目的是通过肺膨胀和通过肺动脉注射放射性不透明聚合物化合物,对早期产后小鼠和成年小鼠的肺动脉网络进行活体可视化。还讨论了铸造组织的潜在应用。

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