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Developmental Biology
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用于微CT成像的成人和早期产后小鼠肺的血管铸造
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Developmental Biology
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JoVE Journal
Developmental Biology
Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging
Please note that all translations are automatically generated.
Click here for the English version.
用于微CT成像的成人和早期产后小鼠肺的血管铸造
DOI:
10.3791/61242-v
•
09:00 min
•
June 20, 2020
•
Russell H. Knutsen
,
Leah M. Gober
,
Joseph R. Sukinik
,
Danielle R. Donahue
,
Elise K. Kronquist
,
Mark D. Levin
,
Sean E. McLean
,
Beth A. Kozel
1
Translational Vascular Medicine Branch, National Heart Lung and Blood Institute
,
National Institutes of Health
,
2
Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke
,
National Institutes of Health
,
3
Division of Pediatric Surgery, Department of Surgery
,
University of North Carolina at Chapel Hill
Chapters
00:04
Introduction
00:52
Pulmonary Artery (PA) Catheterization and Blood Perfusion
03:18
Tracheostomy and Lung Inflation
04:59
Casting the Vasculature
06:44
Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
08:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该技术的目的是通过肺膨胀和通过肺动脉注射放射性不透明聚合物化合物,对早期产后小鼠和成年小鼠的肺动脉网络进行活体可视化。还讨论了铸造组织的潜在应用。
Tags
Vascular Casting
Micro-CT Imaging
Pulmonary Arterial Vasculature
Adult Mouse
Postnatal Mouse
Vascular Morphology
Vascular Architecture
PE10 Tubing
Sodium Nitroprusside
Silk Suture
Right Ventricle
Pulmonary Artery Trunk
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