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Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
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Developmental Biology
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JoVE Journal Developmental Biology
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

English

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12,094 Views

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08:52 min

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March 04, 2015

DOI:

10.3791/52353-v

DOI:
10.3791/52353-v

•

08:52 min
March 04, 2015

•

6 Views
, , ,
1Department of Physiology,University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine,National Yang-Ming University, 3Department of Education and Research,Taipei City Hospital

Summary

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Here we describe a technique for studying hippocampal postnatal neurogenesis using the organotypic slice culture technique. This method allows for in vitro manipulation of adult neurogenesis and allows for the direct application of pharmacological agents to the cultured hippocampus.

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Cite this Article

Mosa, A. J., Wang, S., Tan, Y. F., Wojtowicz, J. M. Organotypic Slice Cultures for Studies of Postnatal Neurogenesis. J. Vis. Exp. (97), e52353, doi:10.3791/52353 (2015).

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