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Evaluación de la oxidación celular utilizando una proteína fluorescente verde sensible al redox específica del compartimento subcelular
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Biochemistry
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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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Evaluación de la oxidación celular utilizando una proteína fluorescente verde sensible al redox específica del compartimento subcelular

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7,065 Views

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06:10 min

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June 18, 2020

DOI:

10.3791/61229-v

DOI:
10.3791/61229-v

•

06:10 min
June 18, 2020

•

4 Views
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1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Summary

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Este protocolo describe la evaluación del estado redox subcelular específico del compartimento dentro de la célula. Una sonda fluorescente sensible al redox permite un análisis ratiométrico conveniente en las células intactas.

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Cite this Article

Tascioglu Aliyev, A., LoBianco, F., Krager, K. J., Aykin-Burns, N. Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein. J. Vis. Exp. (160), e61229, doi:10.3791/61229 (2020).

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