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Biochemistry

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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
 

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Summary
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This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

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Assessment Cellular Oxidation Subcellular Compartment Redox-sensitive Green Fluorescent Protein Protocol Textile Redox State Changes Intracellular Compartment-specific Physiological Mechanisms Pathophysiological Mechanisms Oxidative Stress Non-disruptive Quantification Tiled Eye Sulfide Ratio Intact Cells Ratio Metric Output Signal Expression Levels Photo Bleaching Detection Sensitivities Bacteria In Plants Breast Cancer Cell Line Treat Cells Confluent Cells Trips In EDTA Reaction Arrest Complete Medium Centrifugation Dilute Cells 6 Well Plate Incubation Cell Culture Incubator Viral Row GFP Transduction
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