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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

7,065 Views

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06:10 min

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June 18, 2020

DOI:

10.3791/61229-v

DOI:
10.3791/61229-v

•

06:10 min
June 18, 2020

•

4 Views
, , ,
1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Summary

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This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

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Subcellular CompartmentRedox-sensitive GFPOxidative StressRedox StateRatiometricNon-disruptiveTransductionFlow CytometryHydrogen Peroxide

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Cite this Article

Tascioglu Aliyev, A., LoBianco, F., Krager, K. J., Aykin-Burns, N. Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein. J. Vis. Exp. (160), e61229, doi:10.3791/61229 (2020).

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