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細胞内膜特異的レドックス感受性緑色蛍光タンパク質を用いた細胞酸化の評価
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Biochemistry
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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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細胞内膜特異的レドックス感受性緑色蛍光タンパク質を用いた細胞酸化の評価

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7,065 Views

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06:10 min

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June 18, 2020

DOI:

10.3791/61229-v

DOI:
10.3791/61229-v

•

06:10 min
June 18, 2020

•

4 Views
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1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Summary

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このプロトコルは、細胞内の細胞内区画特異的な酸化還元状態の評価を記述する。レドックス感受性蛍光プローブは、無傷の細胞でのレシオメトリック分析を可能にします。

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Cite this Article

Tascioglu Aliyev, A., LoBianco, F., Krager, K. J., Aykin-Burns, N. Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein. J. Vis. Exp. (160), e61229, doi:10.3791/61229 (2020).

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