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In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
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In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

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5,718 Views

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07:03 min

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May 06, 2020

DOI:

10.3791/61171-v

DOI:
10.3791/61171-v

•

07:03 min
May 06, 2020

•

13 Views
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1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University

Summary

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Aquí se presenta un protocolo para realizar la manipulación genética en el cerebro de hurón embrionario utilizando en la electroporación del útero. Este método permite la focalización de células progenitoras neuronales en el neocórtex in vivo.

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Kalebic, N., Langen, B., Helppi, J., Kawasaki, H., Huttner, W. B. In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation. J. Vis. Exp. (159), e61171, doi:10.3791/61171 (2020).

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