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In Vivo Targeting von neuronalen Vorläuferzellen in Ferret Neocortex durch Utero Elektroporation
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
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In Vivo Targeting von neuronalen Vorläuferzellen in Ferret Neocortex durch Utero Elektroporation

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5,718 Views

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07:03 min

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May 06, 2020

DOI:

10.3791/61171-v

DOI:
10.3791/61171-v

•

07:03 min
May 06, 2020

•

13 Views
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1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University

Summary

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Hier wird ein Protokoll zur genetischen Manipulation im embryonalen Frettchenhirn unter Verwendung der Utero-Elektroporation vorgestellt. Diese Methode ermöglicht die Ausrichtung von neuronalen Vorläuferzellen im Neocortex in vivo.

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Kalebic, N., Langen, B., Helppi, J., Kawasaki, H., Huttner, W. B. In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation. J. Vis. Exp. (159), e61171, doi:10.3791/61171 (2020).

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