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In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
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JoVE 杂志 神经科学
In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

DOI:
10.3791/61171-v

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07:03 min

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May 06, 2020

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, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University

Chapters

  • 00:04Introduction
  • 00:43In Utero Electroporation and Surgical Preparation
  • 02:13In Utero Electroporation
  • 04:45Results: Representative Ferret Neocortex Imaging
  • 06:13Conclusion

Summary

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Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.

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