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In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
 

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Article DOI: 10.3791/61171-v • 07:03 min • May 6th, 2020
May 6th, 2020 •
1,2, 1,3, 1, 4, 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University

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Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.

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In Vivo Targeting Neural Progenitor Cells Ferret Neocortex In Utero Electroporation Genetic Manipulation Animal Model Expansion And Folding Of Neocortex Spatial Specificity Temporal Specificity Neural Stem Cells Brain Development Glass Capillaries Micro Pipette Puller Diameter Adjustment Embryonic Day 33 DNA Concentration Fast Green Anesthesia Sedation Level Isoflurane Adverse Effects Liquid Form Handling Protective Equipment Surgery Canisters Subcutaneous Injection Analgesic Antibiotic Glucose Administration Eye Ointment Application Abdomen Shaving Skin Cleaning And Disinfection
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