Journal
/
/
폐 상피 세포에 있는 슈도모나스 감염에 담배 연기의 효력 공부
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
DOI:

09:15 min

May 11, 2020

, , ,

Chapters

  • 00:04Introduction
  • 00:42Cigarette Smoking Extract (CSE) Preparation
  • 01:39Pseudomonas Culture
  • 02:16Cell Culture and CSE Treatment
  • 03:33Bacterial Infection and Gentamycin Treatment
  • 04:24Determination of Bacterial Concentration Using the Drop Plate Method
  • 04:55RT-qPCR Detection of Bacterial 16S rRNA
  • 07:00Results: CSE-Treatment Increases Bacterial Load
  • 08:23Conclusion

Summary

Automatic Translation

여기에 설명된 담배 연기 추출물이 폐 상피 세포의 세균 성 식민지화에 어떻게 영향을 미치는지 연구하는 프로토콜이 있습니다.

Related Videos

Read Article