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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

5,700 Views

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09:15 min

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May 11, 2020

DOI:

10.3791/61163-v

DOI:
10.3791/61163-v

•

09:15 min
May 11, 2020

•

2 Views
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1Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine,University of Pittsburgh School of Medicine

Summary

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Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.

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Cigarette SmokePseudomonasLung Epithelial CellsBacterial LoadCell CultureSmoke ExtractionBacterial InfectionCell CountingCell SuspensionCell Preparation

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Li, T., Long, C., Fanning, K. V., Zou, C. Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells. J. Vis. Exp. (159), e61163, doi:10.3791/61163 (2020).

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