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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

DOI:
10.3791/61163-v

•

09:15 min

•

May 11, 2020

•

, , ,
1Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 00:42Cigarette Smoking Extract (CSE) Preparation
  • 01:39Pseudomonas Culture
  • 02:16Cell Culture and CSE Treatment
  • 03:33Bacterial Infection and Gentamycin Treatment
  • 04:24Determination of Bacterial Concentration Using the Drop Plate Method
  • 04:55RT-qPCR Detection of Bacterial 16S rRNA
  • 07:00Results: CSE-Treatment Increases Bacterial Load
  • 08:23Conclusion

Summary

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Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.

Tags

Cigarette SmokePseudomonasLung Epithelial CellsBacterial LoadCell CultureSmoke ExtractionBacterial InfectionCell CountingCell SuspensionCell Preparation

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