/
/
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
This content is Free Access.
32,055 Views
•
07:46 min
•
June 05, 2012
DOI:
Summary
Automatically generated
Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.
Read Article
Cite this Article
Kirchner, S., Fothergill, J. L., Wright, E. A., James, C. E., Mowat, E., Winstanley, C. Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung. J. Vis. Exp. (64), e3857, doi:10.3791/3857 (2012).
Copy