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Biochemistry
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基于点击化学的载脂蛋白N-酰基转移酶荧光测定从酶表征到高通量筛选
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Please note that all translations are automatically generated.
Click here for the English version.
基于点击化学的载脂蛋白N-酰基转移酶荧光测定从酶表征到高通量筛选
DOI:
10.3791/61146-v
•
07:37 min
•
May 13, 2020
•
Karine Nozeret
,
Aurélia Pernin
,
Nienke Buddelmeijer
1
Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France
Chapters
00:05
Introduction
00:55
Tube Assay
05:23
Results: Fluorometric Enzyme Assay for Apolipoprotein N-Acyltransferase
07:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一种灵敏的荧光测定法,用于使用二酰基甘油肽和炔烃磷脂作为底物,通过点击化学来监测载脂蛋白N-酰基转移酶活性。
Tags
Click-chemistry
Fluorometric Assay
Apolipoprotein N-acyltransferase
Enzyme Characterization
High-throughput Screening
Integral Membrane Enzyme
Lipid
Detergent
Sonication
Alkyne POPE
FSL-1-biotin
LNT Reaction Buffer
Streptavidin
Azido-FAM
TCEP
TBTA
Copper Sulfate
PBST1
Nonhomogeneous Suspension
Potential Antibiotics
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