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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
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Biochemistry
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JoVE Journal Biochemistry
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:
10.3791/62107-v

•

07:27 min
April 09, 2021

•

9 Views
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1Department of Biology,University of Waterloo

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Alkynyl Fatty Acid AnalogsClick ChemistryFatty Acylated ProteinsFatty Acid LabelingMetabolic LabelingHEK-293 T-cellsBSA-bound Fatty AcidsSaponificationPotassium HydroxideFatty Acid-free BSA

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Liao, L. M. Q., Gray, R. A. V., Martin, D. D. O. Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry. J. Vis. Exp. (170), e62107, doi:10.3791/62107 (2021).

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