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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

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06:07 min

June 07, 2020

DOI:

06:07 min
June 07, 2020

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Summary

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We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.

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