Journal
/
Biochimie
/
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

DOI:
10.3791/61146-v

•

07:37 min

•

May 13, 2020

•

, ,
1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

Chapitres

  • 00:05Introduction
  • 00:55Tube Assay
  • 05:23Results: Fluorometric Enzyme Assay for Apolipoprotein N-Acyltransferase
  • 07:09Conclusion

Summary

Traduction automatique

Traduction automatique

Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.

Tags

Click-chemistryFluorometric AssayApolipoprotein N-acyltransferaseEnzyme CharacterizationHigh-throughput ScreeningIntegral Membrane EnzymeLipidDetergentSonicationAlkyne POPEFSL-1-biotinLNT Reaction BufferStreptavidinAzido-FAMTCEPTBTACopper SulfatePBST1Nonhomogeneous SuspensionPotential Antibiotics

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

A “Dual-Addition” Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Read Article