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Developmental Biology

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从鼠心心室自由壁分离内膜细胞和冠状内皮细胞

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

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从鼠心心室自由壁分离内膜细胞和冠状内皮细胞

16,123 Views

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08:22 min

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April 15, 2020

DOI:

10.3791/61126-v

DOI:

10.3791/61126-v

•

08:22 min

April 15, 2020

•

19 Views

Alyssa Klein^1,2,3, Bethel Bayrau^1,2,3, Yifei Miao^1,2,3,4,5, Mingxia Gu^1,2,3,4,5

¹Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, ²Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, ³Stanford Cardiovascular Institute,Stanford School of Medicine, ⁴Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, ⁵Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center

Summary

我们提出了一种方案，通过消化缓冲液中的连续组织消化、循环离心循环的细胞采集以及使用抗大鼠CD31微珠进行细胞纯化，从大鼠心脏分离内皮和冠状内皮细胞。

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