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April 15, 2020
DOI:
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
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ラット心臓から消化バッファーにおける連続組織消化、再発遠心サイクルからの細胞採取、抗ラットCD31マイクロビーズによる細胞浄化を通じて、内心細胞と冠状動脈内皮細胞を単離するためのプロトコルを提示する。
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Klein, A., Bayrau, B., Miao, Y., Gu, M. Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart. J. Vis. Exp. (158), e61126, doi:10.3791/61126 (2020).
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