Journal
/
Biologia do Desenvolvimento
/
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

DOI:
10.3791/61126-v

•

08:22 min

•

April 15, 2020

•

, , ,
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center

Capítulos

  • 00:04Introduction
  • 01:11Heart Isolation
  • 02:34Endocardial Endothelial Cell (EEC) Digestion
  • 03:34Coronary Endothelial Cell (CEC) Digestion
  • 04:36Endothelial Cell (EC) Sorting
  • 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
  • 07:46Conclusion

Summary

Tadução automática

Tadução automática

We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads.

Tags

Keywords: Endocardial CellsCoronary Endothelial CellsHeartIsolationCell Type-specificGene ExpressionHeart DiseasesTranscriptomicsEpigeneticsSprague Dawley RatsVentricular Free WallDissectionDigestion Buffer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61126?article_type=v

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
check_url/pt/61126?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/pt/61126?article_type=v

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
check_url/pt/61126?article_type=v

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
check_url/pt/61126?article_type=v

Isolation and Characterization of Single Cells from Zebrafish Embryos
check_url/pt/61126?article_type=v

Isolation of Murine Embryonic Hemogenic Endothelial Cells
check_url/pt/61126?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/pt/61126?article_type=v

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
check_url/pt/61126?article_type=v

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
check_url/pt/61126?article_type=v

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Read Article