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Isolamento delle cellule endocartali e coronarie dal muro libero ventricolare del cuore di ratto
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April 15, 2020
DOI:
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
Summary
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Vi presentiamo un protocollo per l'isolamento delle cellule endocardiali e coronariche dai cuori dei ratti attraverso la digestione sequenziale dei tessuti in un buffer di digestione, la raccolta cellulare da cicli di centrifuga ricorrenti e la purificazione cellulare utilizzando microsfere CD31 anti-rat.
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Klein, A., Bayrau, B., Miao, Y., Gu, M. Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart. J. Vis. Exp. (158), e61126, doi:10.3791/61126 (2020).
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