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Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

DOI:
10.3791/61126-v

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08:22 min

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April 15, 2020

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, , ,
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center

章

  • 00:04Introduction
  • 01:11Heart Isolation
  • 02:34Endocardial Endothelial Cell (EEC) Digestion
  • 03:34Coronary Endothelial Cell (CEC) Digestion
  • 04:36Endothelial Cell (EC) Sorting
  • 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
  • 07:46Conclusion

概要

自動翻訳

自動翻訳

We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads.

タグ

Endocardial CellsCoronary Endothelial CellsHeartIsolationCell Type-specificGene ExpressionHeart DiseasesTranscriptomicsエピジェネティクスSprague Dawley RatsVentricular Free WallDissectionDigestion Buffer

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