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Immunology and Infection
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Imagen tridimensional da Vasculatura e Drenagem Linfática Vertebral usando iDISCO+ e Microscopia de Fluorescência de Folha de Luz
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Imagen tridimensional da Vasculatura e Drenagem Linfática Vertebral usando iDISCO+ e Microscopia de Fluorescência de Folha de Luz

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9,578 Views

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10:05 min

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May 22, 2020

DOI:

10.3791/61099-v

DOI:
10.3791/61099-v

•

10:05 min
May 22, 2020

•

20 Views
, ,
1Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, 3Department of Neurology,Yale University School of Medicine

Summary

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Um protocolo é apresentado combinando limpeza tecidual com microscopia de fluorescência de folha de luz (LSFM) para obter imagens tridimensionais e de resolução celular dos vasos linfáticos e linfonodos (LNs) coletando o fluido cefalorraquidal (CSF) e fluido peridural espinhal.

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Cite this Article

Jacob, L., Brito, J., Thomas, J. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. J. Vis. Exp. (159), e61099, doi:10.3791/61099 (2020).

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