Rivista
/
Immunologia e infezioni
/
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

9,589 Views

•

10:05 min

•

May 22, 2020

DOI:

10.3791/61099-v

DOI:
10.3791/61099-v

•

10:05 min
May 22, 2020

•

20 Views
, ,
1Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, 3Department of Neurology,Yale University School of Medicine

Summary

Automatically generated

A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.

Explore More Videos

3D ImagingVertebral Lymphatic VasculatureLymphatic DrainageIDISCO+ ClearingLight Sheet Fluorescence MicroscopySpinal CordMeningesEpidural SpaceDraining Lymph NodesFluorescent TracerPerfusionAnatomical Imaging

Read Article

Cite this Article

Jacob, L., Brito, J., Thomas, J. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. J. Vis. Exp. (159), e61099, doi:10.3791/61099 (2020).

Download .ris file

Copy

Share Video

.

Copy