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Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

9,589 Views

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10:05 min

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May 22, 2020

DOI:

10.3791/61099-v

DOI:
10.3791/61099-v

•

10:05 min
May 22, 2020

•

20 Views
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1Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, 3Department of Neurology,Yale University School of Medicine

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A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.

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Jacob, L., Brito, J., Thomas, J. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. J. Vis. Exp. (159), e61099, doi:10.3791/61099 (2020).

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