ジャーナル
/
免疫学と感染
/
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

DOI:
10.3791/61099-v

•

10:05 min

•

May 22, 2020

•

, ,
1Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, 3Department of Neurology,Yale University School of Medicine

章

  • 00:04Introduction
  • 00:49Intra-Cisterna Magna (ICM) and Thoracolumbar (ThLb) Injection
  • 02:22Perfusion and Tissue Dissection
  • 03:34Whole Mount Vertebral Segment Immunolabeling-Enabled Imaging of Solvent-Cleared Organs-Plus (iDISCO+)
  • 06:14Light Sheet Fluorescence Microscopy (LSFM) Imaging
  • 08:02Results: Representative Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage
  • 09:21Conclusion

概要

自動翻訳

自動翻訳

A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.

タグ

3D ImagingVertebral Lymphatic VasculatureLymphatic DrainageIDISCO+ ClearingLight Sheet Fluorescence MicroscopySpinal CordMeningesEpidural SpaceDraining Lymph NodesFluorescent TracerPerfusionAnatomical Imaging

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Read Article