Journal
/
Immunology and Infection
/
P. aeruginosa Co-cultura 3D infetta di cellule epiteliali bronchiali e macrofagi all'interfaccia aria-liquido per la valutazione preclinica degli anti-infettivi
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Please note that all translations are automatically generated. Click here for the English version.

P. aeruginosa Co-cultura 3D infetta di cellule epiteliali bronchiali e macrofagi all'interfaccia aria-liquido per la valutazione preclinica degli anti-infettivi

italiano

Automatically Generated

10,339 Views

•

10:26 min

•

June 15, 2020

DOI:

10.3791/61069-v

DOI:
10.3791/61069-v

•

10:26 min
June 15, 2020

•

29 Views
, , , , , , , ,
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital

Summary

Automatically generated

Descriviamo un protocollo per un modello di co-coltura tridimensionale di vie aeree infette, utilizzando CFBE41o- cellule, macrofagi THP-1 e Pseudomonas aeruginosa, stabilito presso l'interfaccia aria-liquido. Questo modello fornisce una nuova piattaforma per testare simultaneamente l'efficacia antibiotica, la funzione della barriera epiteliale e i marcatori infiammatori.

Explore More Videos

P. Aeruginosa3D Co-cultureBronchial Epithelial CellsMacrophagesAir-liquid InterfaceAnti-infectivesBiofilmsHost CellsEpithelial BarriersLung InfectionAerosol AdministrationHuman CellsInfection ResearchCell CultivationCFBE41 O Minus CellsCell SeedingPermeable Supports

Read Article

Cite this Article

Montefusco-Pereira, C. V., Horstmann, J. C., Ebensen, T., Beisswenger, C., Bals, R., Guzmán, C. A., Schneider-Daum, N., Carvalho-Wodarz, C. d. S., Lehr, C. P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J. Vis. Exp. (160), e61069, doi:10.3791/61069 (2020).

Download .ris file

Copy

Share Video

.

Copy