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P. アフルギノーサ 抗感染性の前臨床評価のための空気・液体界面における気管支上皮細胞とマクロファージの感染3Dコカルチャー
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Immunology and Infection
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JoVE Journal Immunology and Infection
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
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P. アフルギノーサ 抗感染性の前臨床評価のための空気・液体界面における気管支上皮細胞とマクロファージの感染3Dコカルチャー

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10,311 Views

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10:26 min

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June 15, 2020

DOI:

10.3791/61069-v

DOI:
10.3791/61069-v

•

10:26 min
June 15, 2020

•

29 Views
, , , , , , , ,
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital

Summary

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我々は、CFBE41o-細胞、THP-1マクロファージ、および空気液体界面-で確立された緑膿菌を用いて、感染した気道の3次元共培養モデルのプロトコルについて説明する。このモデルは、抗生物質の有効性、上皮バリア機能、および炎症マーカーを同時にテストするための新しいプラットフォームを提供します。

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Montefusco-Pereira, C. V., Horstmann, J. C., Ebensen, T., Beisswenger, C., Bals, R., Guzmán, C. A., Schneider-Daum, N., Carvalho-Wodarz, C. d. S., Lehr, C. P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J. Vis. Exp. (160), e61069, doi:10.3791/61069 (2020).

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