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Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
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Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

English

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9,905 Views

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09:39 min

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May 30, 2013

DOI:

10.3791/50491-v

DOI:
10.3791/50491-v

•

09:39 min
May 30, 2013

•

5 Views
, ,
1Department of Biological Sciences,Kingsborough Community College, 2Institute for Cellular and Molecular Biology,University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics,Kean University

Summary

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We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.

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Fatty AcidsCholera ToxinHuman Intestinal Epithelial CellsBALB/c Mouse MacrophagesCell Proliferation AssaysMucosal ImmunityCholera Prevention

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Cite this Article

Tamari, F., Tychowski, J., Lorentzen, L. Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages. J. Vis. Exp. (75), e50491, doi:10.3791/50491 (2013).

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