JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
使用χCRAC以高时间分辨率监测体内蛋白质-RNA相互作用动力学
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Please note that all translations are automatically generated.
Click here for the English version.
使用χCRAC以高时间分辨率监测体内蛋白质-RNA相互作用动力学
DOI:
10.3791/61027-v
•
09:15 min
•
May 09, 2020
•
Stuart W. McKellar
,
Ivayla Ivanova
,
Robert W. van Nues
,
Ross A. Cordiner
,
Mehak Chauhan
,
Niki Christopoulou
,
Will Worboys
,
Andrew Langford
,
Torben Heick Jensen
,
Sander Granneman
1
Centre for Engineering Biology
,
University of Edinburgh
,
2
Institute of Cell Biology
,
University of Edinburgh
,
3
Department of Molecular Biology and Genetics
,
Aarhus University
,
4
UVO3 Ltd.
Chapters
00:04
Introduction
00:58
UV Crosslinking and Lysate Production
03:18
Elution and SDS-PAGE
05:24
PCR and Gel Extraction
07:06
Results: Temporal Binding of Nrd1 to its Targets
08:47
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
cDNA的动力学交联和分析是一种允许以高时间分辨率研究活细胞中蛋白质-RNA相互作用动力学的方法。这里详细描述了该方案，包括酵母细胞的生长、UV交联、收获、蛋白质纯化和下一代测序文库的制备步骤。
Tags
Protein-RNA Interaction
Kinetic CRAC
UV Crosslinking
RNA Binding Proteins
RNA Extraction
Cell Harvesting
Time-resolved Analysis
Live Cell Monitoring
Stress Response
Adherent Cells
Microorganisms
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
利用体外荧光共振能量转移研究毫秒时间尺度蛋白质配合物的动力学
在 Vivo 钙成像
中，在 C. elegans
身体壁肌肉
单分子水平标记均匀性的全蛋白质定量
在氧化应激期间细胞粘附和纤维蛋白扩散动力学
使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化
双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学
使用
秀丽隐杆线虫
中的自动包涵体计数监测
体内
蛋白质聚集动力学
使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集
高分辨率中子光谱研究蛋白质和水合水的皮秒纳秒动力学
Read Article
