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Мониторинг динамики взаимодействия белок-РНК in vivo при высоком временном разрешении с использованием χCRAC
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
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Мониторинг динамики взаимодействия белок-РНК in vivo при высоком временном разрешении с использованием χCRAC

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09:15 min

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May 09, 2020

DOI:

10.3791/61027-v

DOI:
10.3791/61027-v

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09:15 min
May 09, 2020

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, , , , , , , , ,
1Centre for Engineering Biology,University of Edinburgh, 2Institute of Cell Biology,University of Edinburgh, 3Department of Molecular Biology and Genetics,Aarhus University, 4UVO3 Ltd.

Tags

Protein-RNA InteractionKinetic CRACUV CrosslinkingRNA Binding ProteinsRNA ExtractionCell HarvestingTime-resolved AnalysisLive Cell MonitoringStress ResponseAdherent CellsMicroorganisms

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McKellar, S. W., Ivanova, I., van Nues, R. W., Cordiner, R. A., Chauhan, M., Christopoulou, N., Worboys, W., Langford, A., Jensen, T. H., Granneman, S. Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC. J. Vis. Exp. (159), e61027, doi:10.3791/61027 (2020).

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