JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
使用基于细胞的雌激素受体对植物雌激素进行筛查β记者分析
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用基于细胞的雌激素受体对植物雌激素进行筛查β记者分析
DOI:
10.3791/61005-v
•
06:07 min
•
June 07, 2020
•
Emily M. Chester
,
Emily Fender
,
Michael D. Wasserman
1
Department of Anthropology
,
Indiana University
Chapters
00:04
Introduction
00:50
Extraction of Potential Plant Phytosteroids
02:29
Human Estrogen Receptor β Transfection Assay
04:25
Results: ERβ Reporter Assay System for Phytoestrogen Activity Screening of Fruits and Vegetables
05:19
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们优化了一种市售雌激素受体β记者检测,用于筛查人类和非人类灵长类动物的食物,以进行雌激素活性活动。我们通过显示已知的雌激素人类食物大豆注册高,而其他食物显示没有活性来验证这一分析。
Tags
Phytoestrogens
Cell-based Assay
Estrogen Receptor
Reporter Assay
Plant Extracts
Lyophilization
Methanol Extraction
DMSO
Sample Preparation
Contamination
Aseptic Technique
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用新型不溶性生色底物检测试剂盒碳水化合物降解酶的高通量筛选
细菌内膜显示筛查抗体片段的图书馆
一种简单的高通量化学筛选方法在
秀丽线虫
中的应用
低亲和力受体-配体相互作用的细胞外蛋白微阵列技术
用于筛查绝经后心血管功能障碍的外源雌激素治疗的活体雌激素缺乏小鼠模型
使用核酸探针进行核酸活性的动力学筛选
用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应
使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化
使用 PRESTO-Tango Assay 在 GPCR-宽范围范围内对 β-Arrestin2 进行配制筛查的平行审讯
基于核磁共振的片段筛选,样品最少,自动化程度最高
Read Article