We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.
Chester, E. M., Fender, E., Wasserman, M. D. Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay. J. Vis. Exp. (160), e61005, doi:10.3791/61005 (2020).