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牵引力显微镜研究B淋巴细胞激活
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Traction Force Microscopy to Study B Lymphocyte Activation
Please note that all translations are automatically generated.
Click here for the English version.
牵引力显微镜研究B淋巴细胞激活
DOI:
10.3791/60947-v
•
09:28 min
•
July 23, 2020
•
Anita Kumari
,
Judith Pineau
,
Ana-Maria Lennon-Duménil
,
Martial Balland
,
Paolo Pierobon
1
Institut Curie, PSL Research University
,
INSERM U932
,
2
Laboratoire Interdisciplinaire de Physique
,
Université Joseph Fourier (Grenoble 1)
Chapters
00:04
Introduction
00:49
Gel Preparation
03:55
Gel Functionalization
04:50
Cell Loading and Imaging
05:47
Analysis
07:01
Results: Information Extracted from Force and Displacement Fields
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,用于在B细胞上进行牵引力显微镜实验。我们描述了软聚丙烯酰胺凝胶的制备及其功能化,以及显微镜下的数据采集和数据分析摘要。
Tags
Traction Force Microscopy
B Lymphocyte Activation
Immune Synapse
Polyacrylamide Gels
Mechanical Capabilities
Integrin
T Cells
Phagocytosis
Salinization
APTMS
Siliconizing Reagent
Gel Polymerization
Ammonium Persulfate
TEMED
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