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Traction Force Microscopy to Study B Lymphocyte Activation
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July 23, 2020
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Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.
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Kumari, A., Pineau, J., Lennon-Duménil, A., Balland, M., Pierobon, P. Traction Force Microscopy to Study B Lymphocyte Activation. J. Vis. Exp. (161), e60947, doi:10.3791/60947 (2020).
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