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Identificazione delle trappole extracellulari neutrophil in Paraffin-Embedded Feline Arterial Thrombi utilizzando la microscopia immunofluorescenza
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March 29, 2020
DOI:
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis
Summary
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Descriviamo un metodo per identificare le trappole extracellulari neutrofiche (NET) nei trombi arteriosi cardiogenici felini fissi e incorporati in parina di formaldeide utilizzando il recupero di antigeni indotti dal calore e un doppio protocollo di immunoetichettatura.
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Duler, L., Nguyen, N., Ontiveros, E., Li, R. H. L. Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy. J. Vis. Exp. (157), e60834, doi:10.3791/60834 (2020).
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