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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Genetics
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JoVE Journal Genetics
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Nederlands

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7,398 Views

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12:19 min

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February 12, 2020

DOI:

10.3791/60824-v

DOI:
10.3791/60824-v

•

12:19 min
February 12, 2020

•

12 Views
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1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics,Icahn School of Medicine at Mount Sinai

Summary

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We bieden een protocol om genen die extracellulaire matrix (ECM) eiwitten coderen in C2C12 myoblasten neer te halen met behulp van small-hairpin (sh) RNA. Gericht op ADAMTSL2 als voorbeeld, beschrijven we de methoden voor de validatie van de knockdown-efficiëntie op het mRNA-, eiwit- en cellulair niveau tijdens C2C12 myoblast tot myotubedifferentiatie.

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Cite this Article

Taye, N., Stanley, S., Hubmacher, D. Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA. J. Vis. Exp. (156), e60824, doi:10.3791/60824 (2020).

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