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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

7,398 Views

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12:19 min

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February 12, 2020

DOI:

10.3791/60824-v

DOI:
10.3791/60824-v

•

12:19 min
February 12, 2020

•

12 Views
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1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics,Icahn School of Medicine at Mount Sinai

Summary

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We provide a protocol to stably knock down genes encoding extracellular matrix (ECM) proteins in C2C12 myoblasts using small-hairpin (sh) RNA. Targeting ADAMTSL2 as an example, we describe the methods for the validation of the knockdown efficiency on the mRNA, protein, and cellular level during C2C12 myoblast to myotube differentiation.

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Cite this Article

Taye, N., Stanley, S., Hubmacher, D. Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA. J. Vis. Exp. (156), e60824, doi:10.3791/60824 (2020).

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